Bacterial pigments are classified as natural products, which are the important classes of highly active natural compounds and also plays a wide range of biological roles such as antioxidant, antibacterial and anticancer etc., Bacterial pigments are categorized as secondary metabolites, these molecules are economically important as a potential applicant in biomedicine. They are widely used in biopharmaceutical industries, due to their needs in various health illnesses in humans. The bacterial secondary metabolites productions are regulated through physical, chemical and biological factors, the scientific based modern tools are playing major role on signaling processing, growth composition regulation and impaired metabolism in bacteria. To know the general mechanisms involved in enhancing the production of bacterial pigments, this review outlines the application of various modern techniques applies to identify the production of secondary metabolites in microorganisms.