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Original Research Article

Anti-Dermatophytic Activity of Titanium Dioxide Nanoparticle
Synthesized using Lawsonia Inermis

Lutfana Banu A1, Soundhari C.2*

1Post Graduate student, 2Associate Professor, Department of Microbiology, Valliammal College for Women, E9, Anna Nagar East, Chennai - 102, Tamil Nadu, India

Ind.J.Applied.Microbiol. 2019 .22(3) : 38-46

*Corresponding author


The development of the green synthesis of nanoparticles using plant extract has received appreciable attention because of the easy preparation methods, less use of chemicals, and is also environment friendly. Hence green synthesis of Titanium dioxide nanoparticle using ethanolic leaf extract of Lawsonia inermis and to evaluate its antidermatophytic activity was attempted in the study. The formation of nanoparticles was indicated a change in color. The morphological image of the TiO2 NPs was observed in a scanning electron microscope, which showed irregular structure with an average size of 71.125nm. The inhibitory activity of synthesized TiO2 NP on the dermatophytes T. mentagrophytes and M.gypseum at concentrations from 2000μg/ml to 31.25μg/ml was evaluated by Disc diffusion method. The ethanolic extract derived TiO2 NP could inhibit T. mentagrophytes significantly with a zone of 30 mm diameter at a MIC of 1000μg/ml, 28mm at 500μg/ml, and 25mm at 250μg/ml. Amphotericin B at a concentration of 1000μg/ml produced an inhibitory zone of 43mm dia. The results hence demonstrate potential anti - dermatophytic activity of ethanolic extract of L. Inermis

Keywords: Lawsonia inermis, Titanium dioxide nanoparticles, antidermatophytic activity, Disc diffusion method

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