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Ashitha.K.Sanuj1*, Sithara Parveen2
*1Assistant Professor, 2Post Graduate student,Department of Microbiology, EMEA college of Arts and Science, Karipur, Kerala- 673647
Ind.J.Applied.Microbiol. 2021 .23(2) : 30-46
Color is the most crucial aspect of any item since it improves its attractiveness and acceptability.Different dyes are available in the market which makes their way to different industries. Syntheticcolors are more reliable and less expensive than natural colors, but due to health hazards, fewremain prohibited. In dye, food, pharmaceutical, cosmetic industries, widely use innocuouspigments from microbes. The work focuses on isolating the pigment-producing bacteria from soiland investigate several parameters that influence pigment synthesis. Bio colorant derivedfrom A1 and A2 were applied on cotton fabric with pigment extracted by the solvent method.Different temperature, pH, incubation, and static and shaking conditions were investigated in thisstudy. Biochemical and Molecular analysis identified both isolates A1 and A2 as Pseudomonasaeruginosa and SalinococcusI roseus. This work demonstrated pigment production was goodunder neutral pH and at shaking conditions. Both isolates showed different incubation times forpigment synthesis. Only Pseudomonas aeruginosa exhibited antibacterial action against the testpathogens E.coli and Staphylococcus aureus.
Keywords: Biocolorant, pigment production, eco-dyeing, antimicrobial activity, Characterization
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