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Original Research Article

Isolation, Optimization and Application of Pigment Producing Bacteria from Soil

Ashitha.K.Sanuj1*, Sithara Parveen2

*1Assistant Professor, 2Post Graduate student,
Department of Microbiology, EMEA college of Arts and Science, Karipur, Kerala- 673647

Ind.J.Applied.Microbiol. 2021 .23(2) : 30-46

*Corresponding author

Abstract:

Color is the most crucial aspect of any item since it improves its attractiveness and acceptability.
Different dyes are available in the market which makes their way to different industries. Synthetic
colors are more reliable and less expensive than natural colors, but due to health hazards, few
remain prohibited. In dye, food, pharmaceutical, cosmetic industries, widely use innocuous
pigments from microbes. The work focuses on isolating the pigment-producing bacteria from soil
and investigate several parameters that influence pigment synthesis. Bio colorant derived
from A1 and A2 were applied on cotton fabric with pigment extracted by the solvent method.
Different temperature, pH, incubation, and static and shaking conditions were investigated in this
study. Biochemical and Molecular analysis identified both isolates A1 and A2 as Pseudomonas
aeruginosa and SalinococcusI roseus. This work demonstrated pigment production was good
under neutral pH and at shaking conditions. Both isolates showed different incubation times for
pigment synthesis. Only Pseudomonas aeruginosa exhibited antibacterial action against the test
pathogens E.coli and Staphylococcus aureus.


Keywords: Biocolorant, pigment production, eco-dyeing, antimicrobial activity, Characterization

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