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Kavitha, G. Priya, G. Banu Priya, R. Balagurunathan and M. Sangeetha
Actinobacterial Research Laboratory, Department of Microbiology, Periyar University, Periyar Palkalai Nagar, Salem – 636 011, Tamil Nadu, India.
Ind.J.Applied.Microbiol. 2017 .20(2) : 1-10
Graphene is a captivated requirement in multiplicity in almost all the fields especially, solar, sensor, battery and biomedical applications due to its superior physcio-chemical and biological properties. Natural sources such as plants, micro and macromolecules and micro organisms are used as reducing agents in graphene nanoparticles synthesis to avoid the hazardous of conventional methods. This study focused isolation of actinobacteria from mushroom associated rhizosphere soil and its role in graphene oxide reduction and bioactive compound production from isolated actinobacteria and analyze its potential. The morphological, microscopical, field emission-scanning electron microscopical view and biochemical identification of isolated actinobaterial strain MAA-1 was observed as Gram-positive, grey color aerial mycelium, non-motile in nature with spiral spore chain morphology and belong to Streptomyces sp. A compound drived from agar surface fermentation of MAA-1 was subjected on methicillin- resistant Staphylococcus aureus and vancomycin resistant Enterococci and found diminutive activity. The intracellular content of Streptomyces sp. changes the brown colour graphene oxide to black colour graphene under specified condition which confirmed the reduction of oxide. and leads to formation of graphene. A disc diffusion method confirms the superior antifungal activity of microbial reduced graphene oxide among the prepared nanoparticles. This is the first report for environmental friendly reduction of graphene oxide using mushroom associated actinobacteria and bioactive compound production to control infections in immune depressive patients. The results suggested that the further optimization, purification, characterization and in vitro analysis of bioactive compound and nano-graphene will be useful for assortment of biomedical and environmental applications.
Keywords: Actinomycetes; Agaricus bisporus, Bioactive Compound, Graphene, Natural products; Streptomycetes.
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