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Original Research Article

Optimization of Biofilm Mediated Antibiotic Resistant Lactobacillus Acidophilus Isolated from Tooth Decay of Rural School Children’s Around Tirupur District

S. Vijayalakshmi, S. Rajasekar and A. Mohankumar*

Division of Microbial Technology, PG and Research Department of Zoology,Chikkanna Govt. Arts College, Tirupur – 641 602, Tamilnadu, India.

Ind.J.Applied.Microbiol. 2017 .20(2) : 11-28

*Corresponding author

Abstract:

Dental caries is one of the main oral problems suffered by the human population. The major constituent of biofilm other than bacterial cells is the Extracellular Polymeric Slime matrix (EPS) which is secreted by the bacterial cells themselves. The cariogenic infection caused by Lactobacillus acidophilus, most common pathogen in human life, causes serious problem among caries patients. Therefore 50 plaque samples were collected from tooth decay of rural school children’s using forceps in and around Tirupur district. The isolates were identified using standard biochemical test. The isolates were screened using microtiter plate assay. Different conditions for better biofilm formation, antibiotic susceptibility of the selected organism was investigated and also the ability of the test pathogen to produce antimicrobial substance. Among the selected isolates, LAVG02, LAVG06, LAVG17, LAVG36, LAVG38, LAVG75 and LAVG85, LAVG92 were found as better biofilm producers and were selected for the detailed study. The strains were cultivated at 30ºC, 37 ºC and 45 ºC at the initial pH of 6.5 for 24 h, with the addition of different concentration (1-10%) of different carbon and nitrogen sources used resulted in different extent of increase in the bacterial growth and biofilm production. In this study glucose and yeast extract induced biofilm formation. It was found that the selected isolate was sensitive to Rifampicin; intermediate to Amoxicillin and resistant to Azithromycin, Vancomycin, Sulphamethizole, Roxithromycin, Cloxacillin, ciprofloxacin and Trimethoprim. Results of this study suggest reducing excessive consumption of sugary foods and drinks will help to prevent the occurrence of dental caries.


Keywords: Dental caries, Biofilm, Culture conditions, Optimization, Lactobacillus acidophilus,antibiotic resistance.

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