Authors are requested to submit the manuscripts only through IJAM web portal, submission directly to journal mail ids will not be considered.
IJAM is now a member of Crossref. Soon articles published in our journal from 2017 will be given DOI number.
• Articles are invited for next issues - Journal frequency thrice in a year - January, May & September

Original Research Article

Extreme Halophilic Archaea Halobacterium sp. SP1 in Bioremediation of Hypersaline Water Polluted with Metal, Metalloid, Organometal and Aromatic Compound

Milind Mohan Naik, Akshata Bagayat and Lakshangy Charya*

Department of Microbiology, Goa University, Goa, India

Ind.J.Applied.Microbiol. 2017 .20(2) : 42-54

*Corresponding author


Extremely halophilic rod shaped, motile prokaryotic microorganism was isolated from sediment of Ribandar salt pan, Goa, India on NTYE agar (25% crude salt) with 500 units/mL penicillin and was designated as SP1. By performing antibiotic sensitivity test, sodium taurocholate sensitivity test, cell lysis in distilled water, glycerol diether lipid moieties in membrane, spectrophotometric analysis of carotenoid pigment and biochemical tests, the isolates SP1 was confirmed to be extreme haloarchaea Halobacterium sp. Extremely haloarchaeal isolate, Halobacterium sp. SP1 could resist 50μM HgCl2 in NGSM broth with 0.1mM MIC (minimum inhibitory concentration). The isolate showed the potential of reducing soluble, toxic selenite into less toxic, insoluble elemental selenium (Se0) which is indicated by change in colony color from red to bright orange. The isolate showed selenite reduction to elemental selenium up to 10 mM with MIC 11 mM. Halobacterium sp. SP1 could degrade up to 1.5 mM sodium benzoate as sole source of carbon on NSM broth (25 % crude salt) and showed 2 mM MIC. Halobacterium sp. SP1 can also capable of utilizing up to 1 mM tributyltin chloride (TBTCl) having 2 mM MIC on NTYE (25% crude salt) agar. Haloarchaea Halobacterium sp. SP1 is multi extremophilic archaea i.e. resistant/degrading multiple pollutants (Hg, Se, sodium benzoate and TBTCl) in hypersaline conditions and is of great importance for bioremediation of hypersaline environments contaminated with multiple pollutants. This is first report of haloarchea Halobacterium sp. resistant to multiple pollutants such as metal, metalloid, organometal and aromatic compound in hypersaline environment.

Keywords: Extreme haloarchaea, salt pan, pollutants, bioremediation.

Download this article as Download

  • Print ISSN : 2249-8400
  • Online ISSN : 2454-289X
  • Issues : 3 per Year
  • Publisher : The Editor in-Chief, IJAM
  • Email :
  • Editor-in-chief: Prof. Dr.Usha Anand Rao

Merits of Publishing in this Journal

  • Wide reach of the paper to the researchers from multidisciplinary background
  • Extension of assistance from experts for quality publication
  • Peer review of paper and needful suggestions
  • Encouragement for innovative research works
  • Prompt publication