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Original Research Article

Altered regulation of Ure A and β-catenin gene expression levels in Helicobacter pylori-associated chronic gastritis

R. Selvi*, Sattar Mohammed Sarnam, S. Kayalvizhi, P.Venkatachalam, A. Rekha, R. Selvaraj, Shanthi Vijayaraghavan

 

Ind.J.Applied.Microbiol. 2017 .20(2) : 74-83

*Corresponding author

Abstract:

Gastritis, an irritation or erosion of the gastric mucosal lining is a distinct clinical entity shown to have an association with chronic peptic ulcer disease and primary gastric cancer. Carcinogenesis is a prolonged and multi-step process that includes initiation, promotion and progression as a result of modification of various cell signaling pathways; alterations in Wnt/ β- catenin pathway is one such feature implicated in gastric cancer. Here we aimed to investigate into the levels of Ure A and β- catenin gene expression in subjects with chronic gastritis and primary gastric ulcer. Fifty gastric biopsy samples were obtained, and RNA was isolated. Following cDNA conversion, the gene expression levels were analyzed using quantitative realtime PCR (qRT-PCR). Among 50 patients, 46% were positive for histopathology and 42% for urease test indicating Helicobacter pylori (H. pylori) infection whereas 38% were positive both by histopathology as well as urease test. The samples that showed positive for urease test and histopathology were subjected to qRT-PCR for expression of Ure A and β-catenin. The results revealed that there are minimal levels of Ure A present in all 19 samples and 21% showed overexpression of β-catenin demonstrating that such detection of H. pylori infection by this approach allows the early diagnosis of gastric cancer.


Keywords: H. pylori, chronic gastritis, Ure A, β- catenin, qRT- PCR

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