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Original Research Article

Isolation and Identification of Endophytic Fungi from Melia azedarach L. of Kalvarayan Hills, Eastern Ghats, Tamil Nadu, India

Selvaraj Kanaga1, Dhandapani Ramamurthi2,K.C.P Rajamanikandan1,3 and D. Arvind Prasanth*1

1Medical Microbiology Laboratory, Department of Microbiology, School of Biosciences, Periyar University, Salem-636011._2Fermentation Technology Laboratory, Department of Microbiology, School of Biosciences, Periyar University, Salem-636011._3 Department of Biotechnology, Jayaram College of Arts and Science, Salem-11

Ind.J.Applied.Microbiol. 2018 .21(1) : 56-68

*Corresponding author

Abstract:

To investigate the Endophytic fungi that are occupied asymptomatically in domestic tissues of all higher plants are of growing interest as promising sources of biologically active agents. The exploitation of novel and eco-friendly secondary metabolites used in medicine, the pharmaceutical industries and agriculture. In this research we select the Melia azedarach L. medicinal plant (exotic tree from Kalvarayan hills) to isolate and identify the endophytic fungi from living apparently symptomless flower, stems, leaves and fruits of medicinal plant will grow very well and is apparently resistant to many pathogenic microorganisms like bacteria, fungi and parasitic infection and it has insecticidal compound. Each segment from the plant of leaves, flowers, fruits and stem each 15 segment of plant parts were be sampled and, total of 47 fungi were isolated. The isolates of endophytic fungi from this plant parts including Cylindrocarpon lichenicola followed by, Chaetomium spp., Curvularia fallax, Fusarium solani, Alternaria alternata, Aspergillus fumigatus, Aspergillus niger, Aspergillus flavus and Aspergillus nidulans. The study the shows the hyphomycetes were more than dematiaceous and mycelia sterilia. The genera of Chaetomium lichenicola and Aspergillus were the most common in that plant.


Keywords:

Medicinal plant, Melia azedarach L, Endophytic fungi, Medicinal properties, Aspergillus sp.

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