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Original Research Article

Study on Bio-film forming bacterial pathogens in diabetic foot ulcer

Sharnita Nadella1, Dr. Sheetal Harakuni2

1.MBBS Student, Jawaharlal Nehru Medical College, KAHER University, Belagavi 590010 2.Professor, Department of Microbiology, Jawaharlal Nehru Medical College, KAHER University, Belagavi 590010

Ind.J.Applied.Microbiol. 2022 .24(1) : 1-8

*Corresponding author

Abstract:

Purpose: Biofilms refer to a group of microorganisms that adhere irreversibly to a surface. Biofilms increase the chronicity of infection and are not penetrable by antibiotics. Strategies preventing biofilm formation in a diabetic foot ulcer patient may help in early healing and cure from infection; along with preventing the severe complications that arise from these infections. Method: The ulcer was cleaned from slough; pus was collected using sterile swab. Gram staining was performed. Samples were inoculated on Blood agar and Mac Conkey medium. Predominant colonies were identified and antibiogram was performed as per standard protocol. Biofilm production by isolated colony was measured by microtiter spectrophotometric method. Result: 32 patients with chronic diabetic foot were included in the study. From these patients, 25 isolates were processed. Three samples grew no bacteria; 4 grew skin commensals, on culture. 32 percent of the isolates were biofilm-producing by the microtiter plate method. Staphylococcus aureus was the predominant pathogen producing biofilm followed by Escherichia coli. Gram positive isolates showed maximum resistance to doxycycline and gram negative isolates to ceftazidime and gentamycin. Conclusion: Biofilm provides a media enabling normally antibiotic sensitive bacteria to exhibit resistance against the same antibiotics. Gram negative bacteria (Escherichia coli predominantly) and Staphylococcus aureus among the gram positive bacteria isolated were identified as predominant biofilm forming isolates.Purpose: Biofilms refer to a group of microorganisms that adhere irreversibly to a surface.Biofilms increase the chronicity of infection and are not penetrable by antibiotics. Strategiespreventing biofilm formation in a diabetic foot ulcer patient may help in early healing and curefrom infection; along with preventing the severe complications that arise from these infections.Method: The ulcer was cleaned from slough; pus was collected using sterile swab. Gram stainingwas performed. Samples were inoculated on Blood agar and Mac Conkey medium. Predominantcolonies were identified and antibiogram was performed as per standard protocol. Biofilmproduction by isolated colony was measured by microtiter spectrophotometric method.Result: 32 patients with chronic diabetic foot were included in the study. From these patients, 25isolates were processed. Three samples grew no bacteria; 4 grew skin commensals, on culture. 32percent of the isolates were biofilm-producing by the microtiter plate method. Staphylococcusaureus was the predominant pathogen producing biofilm followed by Escherichia coli. Grampositive isolates showed maximum resistance to doxycycline and gram negative isolates toceftazidime and gentamycin. Conclusion: Biofilm provides a media enabling normally antibioticsensitive bacteria to exhibit resistance against the same antibiotics. Gram negative bacteria(Escherichia coli predominantly) and Staphylococcus aureus among the gram positive bacteriaisolated were identified as predominant biofilm forming isolates.


Keywords: Diabetic foot ulcer, antibiotic sensitivity resistance, biofilm

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